Lambeth council is managing a campaign to cut HIV infection across the capital, launched in May.
The £3.4 million three-year programme, funded by all 32 London boroughs and the City of London, is a direct response to the fact that London has the highest prevalence of HIV in England.
Advertising for the Do It London campaign is appearing on more than 200 phone boxes across the capital.
There will also be publicity at Gay Pride and Notting Hill Carnival, as well as during the Christmas and New Year party season and National HIV Prevention Week in November.
Cllr Teresa O’Neill, London Councils’ Executive member for health, said: “London boroughs are determined to reduce the prevalence of HIV and the launch of the ‘Do It London’ campaign marks an important step in raising awareness of testing across the capital.
“Working with the boroughs, London Councils will be pressing government to support the fight against HIV through allocating funding in a way which properly reflects the scale of this complex and urgent challenge.”
Research shows 33,863 Londoners are living with diagnosed HIV and 2,122 of the 6,000 new HIV diagnoses in 2013 were in London.